Cell Engineering Senior Scientist

Johnson & Johnson is currently seeking a Cell Engineering Senior Scientist to join our Cell Engineering & Analytical Sciences group within the API-Proteins organization located in Spring House, PA.

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As part of this role, you will be responsible for developing assays to understand monoclonal antibodies expression systems using non-natural amino acids. Your experience in RNA, DNA, and protein analysis techniques will be valuable in optimizing our non-natural amino acid platform and facilitating the production of high-quality biotherapeutics.

Join our pioneering mass spectrometry laboratory and gain access to the latest instruments, robotic workbenches for automated sample handling, and powerful bioinformatic software and cloud computing resources. Working in a dynamic and interdisciplinary setting, you will collaborate with multi-functional teams, including those specializing in cell line development and cell culture process development. In this role, you will offer expert guidance on extensive Mass Spec characterization of biotherapeutics and explore various aspects of cellular and protein expression within cells. Through your valuable contributions, you will actively contribute to the advancement of our biopharmaceutical pipeline.

Joining our team as a Cell Engineering Senior Scientist specializing in cell engineering offers a unique and exciting opportunity to contribute to the development of innovative biopharmaceutical products. If you are passionate about protein characterization, oligonucleotide analysis, and protein expression, and thrive in an exciting and collaborative scientific environment, we encourage you to apply and become part of our team.

Key Responsibilities:

• Develop and implement mass spectrometry assays to optimize protein expression levels, protein quality, and quantitation of nnAA orthogonal elements (such as tRNA and RNA synthetase). Ensure accuracy and success of MS experiments through expertise in sample preparation.
• Use advanced liquid chromatography-mass spectrometry (LC-MS/MS) technologies for protein identification and intra-cellular quantification.
• Employ top-down mass spectrometry methods to quantitate truncated protein both inter- and intra-cellularly.
• Analyze antibodies with nnAA incorporation using the peptide mapping technique to identify the site of insertion and perform sequence variant analysis.
• Collaborate with other functions and teams to design experiments that address critical questions.
• Troubleshoot technical issues and propose innovative solutions to enhance analytical capabilities.
• Stay up to date with the latest advancements in oligonucleotide, protein quantitation, and cell engineering. Develop new methods and publish findings in peer-reviewed journals.
• Prepare and present scientific findings and recommendations to internal customers, project teams, and senior management.

Qualifications

Required:

• Ph. D. in life sciences or a related field with 0-3 years of experience OR a Master's degree with 4-6 years of experience
• Strong background and hands-on experience in oligonucleotide techniques, peptide quantitation, sample preparation, mass spectrometry-based analysis, and interpretation.
• Knowledge of tRNA extraction techniques and mass spectrometry characterization of intracellular protein expression components.
• In-depth experience operating mass spectrometers from all vendors and optimizing instrument parameters and data analysis.
• Proven track record of working independently and collaboratively in a multidisciplinary team environment.
• Excellent problem-solving skills and attention to detail.
• Strong written and oral communication skills, with the ability to effectively summarize and communicate in-depth MS results to multi-disciplinary teams.

Preferred:

• Experience in oligonucleotide mass spectrometry workflows and data analysis.
• Experience working with non-natural amino acids (nnAA)
• Advanced knowledge of protein and genetic components required for expression systems.
• Proven experience in mass spectrometry protein characterization.
• Experience with protein quantitation via Multiple Reaction Monitoring MS (Mass Spectrometry) methods.
• Prior experience in the biopharmaceutical industry or related fields.
• Solid understanding of mass spectrometry and MS sample preparation.
• This role is based in Spring House, PA and may require 5% travel.

Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.

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